Composite

Part:BBa_K2120423

Designed by: Lirong Zhao   Group: iGEM16_BIT-China   (2016-10-13)


B0015+cI+B0034+J23116+pR+B0032+mRFP+B0015

This is one of our application circuit.


<Description>
This device is similar to our project final gene circuit using relationship of inhibitor-promoter to start downstream gene’s transcription.We use RFP as a report gene. The report gene could indicate a safety condition when killer gene express.
In order to adjust the repressing efficiency, we choose medium weak constitutive promoter J23116 and strong RBS B0034 to express inhibitor gene cI. Meanwhile, we also constructed more circuits which have different combine with promoter, RBS and inhibitor. More detail see in our Wiki([http://2016.igem.org/Team:BIT-China/Parts Parts Submission])


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 941
    Illegal NheI site found at 964
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1614
    Illegal AgeI site found at 1726
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
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